Elucidate software4/2/2023 In both symbiotic models, the microbial symbiotic partner can show a variable degree of host specificity (resulting from multiple interactions involving signaling among bacteria and host plants): some strains establish highly specific interactions with their host, while others are versatile and infect a large spectrum of host plants. The Sp+ genomes were also characterized by a loss of genetic and functional paralogs, highlighting a reduction in functional redundancy (e.g., hup genes) or a possible loss of function related to a saprophytic lifestyle (e.g., genes involved in gas vesicle formation or recycling of nutrients). The lost genes were related to saprophytic life (transcriptional factors, transmembrane and secreted proteins), reinforcing the proposed status of Sp+ as obligatory symbiont. A total of 88 protein families were lost in the Sp+ genomes. Within “ Alnus-infective strains”, Sp+ Frankia genomes were compared to Sp− genomes in order to elucidate the narrower host specificity of Sp+ strains (i.e., Sp+ strains being capable of in planta sporulation, unlike Sp− strains). Several genes were specifically found in these strains, including an agmatine deiminase which could possibly be involved in various functions as access to nitrogen sources, nodule organogenesis or plant defense. The determinants of host specificity were first explored for “ Alnus-infective strains” (i.e., Frankia strains belonging to Cluster Ia). Presentation of the MS/MS spectra in a 'tree' format greatly aided in postulating fragmentation schemes which were consistent with the observed electron impact results since the TSQ 'tree' program is able to display the entire three dimensional MS/MS data field in a convenient and controllable format.The present study aimed to use comparative genomics to explore the relationships between Frankia and actinorhizal plants using a data set made of 33 Frankia genomes. A mechanism has been proposed for the formation of the (M minus 102)** plus ion which includes a cyclic product, analogous to the one formed after elimination of ammonia from the ethyl ester of lysine. Presentation of the MS/MS spectra in a 'tree' format greatly aided in postulating fragmentation schemes which were consistent with the observed electron impact results since the TSQ 'tree' program is able to display the entire three dimensional MS/MS data field in a convenient and controllable format.ĪB - MS/MS evaluation of the di-acetamide series and corresponding deutero-acetyl analogs indicated that a step-wise process involving sequential loss of acetyl, ammonia and ketene groups, as well as concerted pathways for the combined loss of these moieties was operative during fragmentation. N2 - MS/MS evaluation of the di-acetamide series and corresponding deutero-acetyl analogs indicated that a step-wise process involving sequential loss of acetyl, ammonia and ketene groups, as well as concerted pathways for the combined loss of these moieties was operative during fragmentation. T1 - NEW MS/MS SOFTWARE TOOLS FOR ELUCIDATION OF FRAGMENTATION MECHANISMS IN DI-ACETAMIDES.
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